Hotspot

ndhK-ndhC

LSC · 76540-76609 · 70 bp

Back to Rorippa

Pi 0.0357

MarkerSeek score 77.50

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 70
variable_sites: 4
pi: 0.0357
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	GGCTGGAAAGTCTTGACCAA	CATGGCGAAAAGGAGCATTG	50.0	50.0	58.0	58.1	197-211 bp	1.0	83.9
ndhK-ndhC_p2	GGCTGGAAAGTCTTGACCAA	TGGGCAATGAGTTTCGATGT	50.0	45.0	58.0	57.8	285-299 bp	1.0	81.6
ndhK-ndhC_p3	GGCTGGAAAGTCTTGACCAA	TGTACTGGGGGTATCTGCTT	50.0	50.0	58.0	57.7	268-282 bp	1.0	81.2
ndhK-ndhC_p4	GGCTGGAAAGTCTTGACCAA	GAGTTTCGATGTACTGGGGG	50.0	55.0	58.0	57.7	277-291 bp	1.0	80.8
ndhK-ndhC_p5	GGCTGGAAAGTCTTGACCAA	AGTTTCGATGTACTGGGGGT	50.0	50.0	58.0	58.3	276-290 bp	1.0	80.5