Hotspot

trnF-GAA-ndhJ

LSC · 74697-75281 · 585 bp

Back to Rorippa

Pi 0.0345

MarkerSeek score 74.90

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 585
variable_sites: 32
pi: 0.0345
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CATCCACGACTGAAACGGAT	50.0	50.0	58.1	58.0	718-731 bp	1.0	85.0
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CCCGAAAGTTGGATAGGGTG	50.0	55.0	58.1	58.0	691-704 bp	1.0	85.0
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGATAGGGTGG	50.0	55.0	58.1	58.0	690-703 bp	1.0	85.0
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	CCTAATGCCCGAAAGTTGGA	50.0	50.0	58.1	57.9	698-711 bp	1.0	84.3
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	TCCTAATGCCCGAAAGTTGG	50.0	50.0	58.1	57.9	699-712 bp	1.0	84.3