Hotspot

trnD-GUC-trnY-GUA

LSC · 56380-56807 · 428 bp

Back to Rorippa

Pi 0.0284

MarkerSeek score 67.30

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 428
variable_sites: 20
pi: 0.0284
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.5	57.8	493-498 bp	1.0	79.6
trnD-GUC-trnY-GUA_p2	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.5	57.8	492-497 bp	1.0	79.6
trnD-GUC-trnY-GUA_p3	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.5	58.4	514-519 bp	1.0	78.5
trnD-GUC-trnY-GUA_p4	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.5	57.6	513-518 bp	1.0	78.3
trnD-GUC-trnY-GUA_p5	CGGTGCTCTGACCAATTGAA	CGGACTGTAAATTCGTTGGC	50.0	50.0	58.5	57.5	509-514 bp	1.0	77.2