Hotspot

trnH-GUG-psbA

LSC · 26512-26826 · 315 bp

Back to Rorippa

Pi 0.0414

MarkerSeek score 70.00

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 315
variable_sites: 24
pi: 0.0414
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	GCTGTTGAGGCTCCATCTAC	50.0	55.0	57.9	58.1	373-391 bp	1.0	83.9
trnH-GUG-psbA_p2	ACTGCCTTAATCCACTTGGC	GCTGTTGAGGCTCCATCTAC	50.0	55.0	58.2	58.1	374-392 bp	1.0	83.7
trnH-GUG-psbA_p3	AATCCACTTGGCTACATCCG	GCTGTTGAGGCTCCATCTAC	50.0	55.0	57.7	58.1	366-384 bp	1.0	82.5
trnH-GUG-psbA_p4	CTGCCTTAATCCACTTGGCT	TGCTGTTGAGGCTCCATCTA	50.0	50.0	57.9	58.4	374-392 bp	1.0	80.9
trnH-GUG-psbA_p5	ACTGCCTTAATCCACTTGGC	TGCTGTTGAGGCTCCATCTA	50.0	50.0	58.2	58.4	375-393 bp	1.0	80.7