Hotspot

rps16

LSC · 5242-6333 · 1092 bp

Back to Roldana

Pi 0.00826

MarkerSeek score 63.80

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1092
variable_sites: 9
pi: 0.00826
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TCGTTTGATTAGGGTAAAGTTCCT	GTCTATGTACCGAGCGAACC	37.5	55.0	57.9	57.9	1196-1199 bp	1.0	46.9
rps16_p2	TCGTTTGATTAGGGTAAAGTTCCT	CCGAGCGAACCAATGACTAT	37.5	50.0	57.9	57.8	1187-1190 bp	1.0	46.1
rps16_p3	TCGTTTGATTAGGGTAAAGTTCCT	TCTATGTACCGAGCGAACCA	37.5	50.0	57.9	58.2	1195-1198 bp	1.0	45.9
rps16_p4	TCGTTTGATTAGGGTAAAGTTCCT	ATGTACCGAGCGAACCAATG	37.5	50.0	57.9	58.3	1192-1195 bp	1.0	45.1
rps16_p5	TCGTTTGATTAGGGTAAAGTTCC	GTCTATGTACCGAGCGAACC	39.1	55.0	56.5	57.9	1196-1199 bp	1.0	44.2