Hotspot

clpP

LSC · 68985-70995 · 2011 bp

Back to Roldana

Pi 0.0159

MarkerSeek score 61.60

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2011
variable_sites: 32
pi: 0.0159
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	ATTGACGCGAAATACAGGCT	GGCTTTGTTACGTTTCCACA	45.0	45.0	58.0	56.9	2097-2104 bp	1.0	71.9
clpP_p2	CTCGGCAGGGTTAACTTCAA	GGCTTTGTTACGTTTCCACA	50.0	45.0	57.8	56.9	2124-2131 bp	1.0	70.7
clpP_p3	AACTCGGCAGGGTTAACTTC	GGCTTTGTTACGTTTCCACA	50.0	45.0	57.8	56.9	2126-2133 bp	1.0	70.7
clpP_p4	ATTGACGCGAAATACAGGCT	AGGCTTTGTTACGTTTCCACA	45.0	42.9	58.0	58.4	2098-2105 bp	1.0	70.2
clpP_p5	ATTGACGCGAAATACAGGCT	AGGCTTTGTTACGTTTCCAC	45.0	45.0	58.0	56.6	2098-2105 bp	1.0	69.6