Hotspot

rpl32-ndhF

SSC · 122891-123955 · 1065 bp

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Pi 0.0181

MarkerSeek score 58.30

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1065
variable_sites: 19
pi: 0.0181
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	AACGCTGCCCAATATCCTTT	CCACGCATATTGATATATCTGTTCC	45.0	40.0	57.9	57.8	1166-1178 bp	1.0	42.5
rpl32-ndhF_p2	ACGCTGCCCAATATCCTTTC	CCACGCATATTGATATATCTGTTCC	50.0	40.0	58.3	57.8	1165-1177 bp	1.0	42.5
rpl32-ndhF_p3	GCTGCCCAATATCCTTTCCT	CCACGCATATTGATATATCTGTTCC	50.0	40.0	57.6	57.8	1163-1175 bp	1.0	42.5
rpl32-ndhF_p4	ACAAAGCTTTTAACGCTGCC	CCACGCATATTGATATATCTGTTCC	45.0	40.0	57.9	57.8	1177-1189 bp	1.0	42.4
rpl32-ndhF_p5	CAAAGCTTTTAACGCTGCCC	CCACGCATATTGATATATCTGTTCC	50.0	40.0	58.6	57.8	1176-1188 bp	1.0	42.4