Hotspot

ndhI-ndhG

SSC · 116961-117321 · 361 bp

Back to Roldana

Pi 0.0277

MarkerSeek score 69.70

Primer available yes

Label ndhI-ndhG

Feature Metadata

label_name: ndhI-ndhG
feature_type: igs
region: SSC
length_bp: 361
variable_sites: 10
pi: 0.0277
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhG_p1	TCAAATTGCTTTGGGTCGTT	TCCCAATGAAAAGGCGGAAT	40.0	45.0	56.4	57.8	569 bp	1.0	67.1
ndhI-ndhG_p2	ATCAAATTGCTTTGGGTCGT	TCCCAATGAAAAGGCGGAAT	40.0	45.0	56.2	57.8	570 bp	1.0	65.3
ndhI-ndhG_p3	TCAAATTGCTTTGGGTCGTTT	TCCCAATGAAAAGGCGGAAT	38.1	45.0	57.2	57.8	569 bp	1.0	65.1
ndhI-ndhG_p4	AATCAAATTGCTTTGGGTCGT	TCCCAATGAAAAGGCGGAAT	38.1	45.0	57.0	57.8	571 bp	1.0	63.3
ndhI-ndhG_p5	ATCAAATTGCTTTGGGTCGTT	TCCCAATGAAAAGGCGGAAT	38.1	45.0	57.0	57.8	570 bp	1.0	63.3