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psbT-psbN

LSC · 73269-73337 · 69 bp

Back to Roldana

Pi 0.188

MarkerSeek score 76.70

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 69
variable_sites: 12
pi: 0.188
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	GAGAACCGCCGAAAATTTCA	TCGAGGAACATGGGGACTAA	45.0	50.0	57.0	57.8	121 bp	0.5	50.2
psbT-psbN_p2	GAGAACCGCCGAAAATTTCA	TTCGAGGAACATGGGGACTA	45.0	50.0	57.0	57.8	122 bp	0.5	50.2
psbT-psbN_p3	AGAACCGCCGAAAATTTCAAC	TCGAGGAACATGGGGACTAA	42.9	50.0	58.0	57.8	120 bp	0.5	50.0
psbT-psbN_p4	GAACCGCCGAAAATTTCAACT	TCGAGGAACATGGGGACTAA	42.9	50.0	58.0	57.8	119 bp	0.5	50.0
psbT-psbN_p5	GAACCGCCGAAAATTTCAACT	TTCGAGGAACATGGGGACTA	42.9	50.0	58.0	57.8	120 bp	0.5	50.0