Hotspot

rps18-rpl20

LSC · 67285-67601 · 317 bp

Back to Roldana

Pi 0.0285

MarkerSeek score 69.50

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 317
variable_sites: 9
pi: 0.0285
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	AACCGAGTCGACCACTAGAA	TCTGTACAAGAGACAGTTGCT	50.0	42.9	58.1	56.6	477-478 bp	1.0	61.8
rps18-rpl20_p2	AAGAACCGAGTCGACCACTA	TCTGTACAAGAGACAGTTGCT	50.0	42.9	58.1	56.6	480-481 bp	1.0	61.8
rps18-rpl20_p3	TTGAAAGAACCGAGTCGACC	TCTGTACAAGAGACAGTTGCT	50.0	42.9	57.9	56.6	484-485 bp	1.0	61.4
rps18-rpl20_p4	AACCGAGTCGACCACTAGAA	TCTGTACAAGAGACAGTTGCTT	50.0	40.9	58.1	57.3	477-478 bp	1.0	59.5
rps18-rpl20_p5	AACCGAGTCGACCACTAGAA	TGTACAAGAGACAGTTGCTTCT	50.0	40.9	58.1	57.3	475-476 bp	1.0	59.5