Hotspot

ycf3-trnS-GGA

LSC · 43955-44834 · 880 bp

Back to Roldana

Pi 0.0193
MarkerSeek score 69.90
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
880
variable_sites
17
pi
0.0193
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TTCTCCTGAAGTTGTCGGAA ATGCTACGCTTTCAACCACT 45.0 45.0 56.4 57.8 1009 bp 1.0 67.2
ycf3-trnS-GGA_p2 TTTCTCCTGAAGTTGTCGGA ATGCTACGCTTTCAACCACT 45.0 45.0 56.4 57.8 1010 bp 1.0 67.2
ycf3-trnS-GGA_p3 TTCTCCTGAAGTTGTCGGAA GGATTCGAACCCTCGGTAAG 45.0 55.0 56.4 57.8 1052 bp 1.0 66.9
ycf3-trnS-GGA_p4 TTTCTCCTGAAGTTGTCGGA GGATTCGAACCCTCGGTAAG 45.0 55.0 56.4 57.8 1053 bp 1.0 66.9
ycf3-trnS-GGA_p5 TTCTCCTGAAGTTGTCGGAA CCAATGCTACGCTTTCAACC 45.0 50.0 56.4 57.7 1012 bp 1.0 66.6