Hotspot

psaA-ycf3

LSC · 41341-42026 · 686 bp

Back to Roldana

Pi 0.0204

MarkerSeek score 70.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 686
variable_sites: 14
pi: 0.0204
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GAGAAATGACCCGGTCTAGC	AGCCGCTGAGTATTGGAAAC	55.0	50.0	57.8	58.3	878 bp	1.0	77.4
psaA-ycf3_p2	GAGAAATGACCCGGTCTAGC	CAAGCCGCTGAGTATTGGAA	55.0	50.0	57.8	58.3	880 bp	1.0	77.4
psaA-ycf3_p3	GAGAAATGACCCGGTCTAGC	AAGCCGCTGAGTATTGGAAA	55.0	45.0	57.8	57.5	879 bp	1.0	75.5
psaA-ycf3_p4	TTTTACTTCTGGTTCCGGCG	AGCCGCTGAGTATTGGAAAC	50.0	50.0	58.5	58.3	813 bp	1.0	75.4
psaA-ycf3_p5	TTTTACTTCTGGTTCCGGCG	CAAGCCGCTGAGTATTGGAA	50.0	50.0	58.5	58.3	815 bp	1.0	75.4