Hotspot

atpH-atpF

LSC · 26453-26841 · 389 bp

Back to Roldana

Pi 0.0154

MarkerSeek score 63.20

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 389
variable_sites: 6
pi: 0.0154
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	CGAGGCGGAGGGAAAAATAA	TGGCCCAAAGAAACGAAAGA	50.0	45.0	58.0	57.9	545 bp	1.0	80.1
atpH-atpF_p2	CGAGGCGGAGGGAAAAATAA	ATTAAACCCGAAACTCCCGG	50.0	50.0	58.0	57.9	576 bp	1.0	79.6
atpH-atpF_p3	CGAGGCGGAGGGAAAAATAA	GTGGCCCAAAGAAACGAAAG	50.0	50.0	58.0	57.9	546 bp	1.0	79.5
atpH-atpF_p4	CGAGGCGGAGGGAAAAATAA	AGTGGCCCAAAGAAACGAAA	50.0	45.0	58.0	58.2	547 bp	1.0	78.8
atpH-atpF_p5	GCCTGGTTGTAGCATTAGCA	TGGCCCAAAGAAACGAAAGA	50.0	45.0	58.2	57.9	474 bp	1.0	78.5