Hotspot

trnC-GCA-petN

LSC · 9506-10303 · 798 bp

Back to Roldana

Pi 0.0263
MarkerSeek score 74.00
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
798
variable_sites
21
pi
0.0263
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 GTTGTGTATCGTTTTGGCGG AGCCCAAGCGAGACTTACTA 50.0 50.0 58.0 58.2 921-926 bp 1.0 80.4
trnC-GCA-petN_p2 TTTCCCCAGTTCAAATCCGG AGCCCAAGCGAGACTTACTA 50.0 50.0 58.1 58.2 864-869 bp 1.0 80.2
trnC-GCA-petN_p3 GGGGGACTGCAAATCCTTTT AGCCCAAGCGAGACTTACTA 50.0 50.0 58.4 58.2 882-887 bp 1.0 77.9
trnC-GCA-petN_p4 TGTTGTGTATCGTTTTGGCG AGCCCAAGCGAGACTTACTA 45.0 50.0 57.3 58.2 922-927 bp 1.0 75.3
trnC-GCA-petN_p5 GTTGTGTATCGTTTTGGCGG GCCCAAGCGAGACTTACTAT 50.0 50.0 58.0 57.1 920-925 bp 1.0 74.4