Hotspot

ycf1

SSC · 125886-131345 · 5460 bp

Pi 0.00259

MarkerSeek score 41.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5460
variable_sites: 26
pi: 0.00259
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTCTACGACGTCTGGGAGAT	TCAAGAGCAAGAAAGACGCA	55.0	45.0	58.0	57.8	495 bp	1.0	78.9
ycf1_p2	CTCTACGACGTCTGGGAGAT	AGCAAGAAAGACGCAGAACT	55.0	45.0	58.0	57.8	490 bp	1.0	78.8
ycf1_p3	CTCTACGACGTCTGGGAGAT	GCAAGAAAGACGCAGAACTG	55.0	50.0	58.0	57.7	489 bp	1.0	78.3
ycf1_p4	CTCTACGACGTCTGGGAGAT	AGATTACACGGGAGCAGACA	55.0	50.0	58.0	58.4	542 bp	1.0	77.1
ycf1_p5	TTCTCTACGACGTCTGGGAG	TCAAGAGCAAGAAAGACGCA	55.0	45.0	58.3	57.8	497 bp	1.0	77.1