Hotspot

ccsA

SSC · 116337-117332 · 996 bp

Back to Rohdea

Pi 0
MarkerSeek score 28.00
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
SSC
length_bp
996
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
28.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 GAGTGGCGGCATACTGTAAA ACGATGTTATTATGTATTCCTCATGG 50.0 34.6 58.0 57.2 1100 bp 1.0 40.0
ccsA_p2 GAGTGGCGGCATACTGTAAA CGATGTTATTATGTATTCCTCATGGA 50.0 34.6 58.0 57.0 1099 bp 1.0 40.0
ccsA_p3 GACACGCTGCTCTTAGGAAG ACGATGTTATTATGTATTCCTCATGG 55.0 34.6 58.4 57.2 1148 bp 1.0 40.0
ccsA_p4 AGTGGCGGCATACTGTAAAA ACGATGTTATTATGTATTCCTCATGG 45.0 34.6 57.5 57.2 1099 bp 1.0 40.0
ccsA_p5 GAGTGGCGGCATACTGTAAA CGATGTTATTATGTATTCCTCATGG 50.0 36.0 58.0 55.7 1099 bp 1.0 40.0