Hotspot

petA-psbJ

LSC · 64122-65213 · 1092 bp

Back to Rohdea

Pi 0.00549
MarkerSeek score 53.20
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1092
variable_sites
12
pi
0.00549
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGCATCCGTTATTTTGGCAC CCGGATTGGGTTCATCTCTG 50.0 55.0 58.1 58.0 1200 bp 1.0 79.8
petA-psbJ_p2 GGCCTTTTGTTCTTCTTGGC CCGGATTGGGTTCATCTCTG 50.0 55.0 57.8 58.0 1217 bp 1.0 79.2
petA-psbJ_p3 GGCATCCGTTATTTTGGCAC TCCGGATTGGGTTCATCTCT 50.0 50.0 58.1 58.1 1201 bp 1.0 79.2
petA-psbJ_p4 GGCCTTTTGTTCTTCTTGGC TCCGGATTGGGTTCATCTCT 50.0 50.0 57.8 58.1 1218 bp 1.0 78.6
petA-psbJ_p5 TGTTCTTCTTGGCATCCGTT CCGGATTGGGTTCATCTCTG 45.0 55.0 57.7 58.0 1210 bp 1.0 78.3