Hotspot

rbcL-accD

LSC · 57783-58471 · 689 bp

Back to Rohdea

Pi 0.00148
MarkerSeek score 52.20
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
689
variable_sites
2
pi
0.00148
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 CGCTGCTTGTGAAGTATGGA CCGGTATGTCCAACAGCATT 50.0 50.0 58.3 58.2 852-866 bp 1.0 77.3
rbcL-accD_p2 CGCTGCTTGTGAAGTATGGA CTTCCACCGGTATGTCCAAC 50.0 55.0 58.3 58.3 858-872 bp 1.0 77.2
rbcL-accD_p3 AAATGGAGCCCTGAACTAGC CCGGTATGTCCAACAGCATT 50.0 50.0 57.6 58.2 872-886 bp 1.0 76.0
rbcL-accD_p4 AAATGGAGCCCTGAACTAGC CTTCCACCGGTATGTCCAAC 50.0 55.0 57.6 58.3 878-892 bp 1.0 75.9
rbcL-accD_p5 CGCTGCTTGTGAAGTATGGA ACTTCCACCGGTATGTCCAA 50.0 50.0 58.3 58.6 859-873 bp 1.0 74.1