Hotspot

trnT-GGU-psbD

LSC · 32463-33221 · 759 bp

Back to Rohdea

Pi 0.0022
MarkerSeek score 51.10
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
759
variable_sites
3
pi
0.0022
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GGACCAGCCTACAAAAACGA 55.0 50.0 58.1 58.1 918-919 bp 1.0 79.0
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GGACCAGCCTACAAAAACGA 50.0 50.0 58.1 58.1 921-922 bp 1.0 78.8
trnT-GGU-psbD_p3 GGCGTAAGTCATCGGTTCAA GGACCAGCCTACAAAAACGA 50.0 50.0 58.3 58.1 895-896 bp 1.0 77.3
trnT-GGU-psbD_p4 GGCCCTTTTAACTCAGTGGT GGACCAGCCTACAAAAACGA 50.0 50.0 57.7 58.1 933-934 bp 1.0 77.2
trnT-GGU-psbD_p5 TGGTAGAGTAACGCCATGGT GGACCAGCCTACAAAAACGA 50.0 50.0 58.4 58.1 917-918 bp 1.0 76.0