Hotspot

psbK-psbI

LSC · 7731-8135 · 405 bp

Back to Rohdea

Pi 0.00908
MarkerSeek score 51.70
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
405
variable_sites
6
pi
0.00908
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCTGCTGTAAG GGCCCGGATCGTTAGATAAG 50.0 55.0 57.9 57.6 521-523 bp 1.0 77.9
psbK-psbI_p2 CTTTTGTTTGGCAAGCTGCT GGCCCGGATCGTTAGATAAG 45.0 55.0 58.1 57.6 526-528 bp 1.0 77.8
psbK-psbI_p3 TGGCAAGCTGCTGTAAGTTT GGCCCGGATCGTTAGATAAG 45.0 55.0 58.3 57.6 518-520 bp 1.0 75.8
psbK-psbI_p4 TTGGCAAGCTGCTGTAAGTT GGCCCGGATCGTTAGATAAG 45.0 55.0 58.3 57.6 519-521 bp 1.0 75.8
psbK-psbI_p5 TTTGGCAAGCTGCTGTAAGT GGCCCGGATCGTTAGATAAG 45.0 55.0 58.3 57.6 520-522 bp 1.0 75.8