Hotspot

rps16-trnQ-UUG

LSC · 5836-7127 · 1292 bp

Back to Rohdea

Pi 0.012
MarkerSeek score 51.60
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1292
variable_sites
24
pi
0.012
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GGTTCGAATCCTTCCGTCC 45.0 57.9 58.1 57.9 1363-1367 bp 1.0 71.9
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GTTCGAATCCTTCCGTCCC 45.0 57.9 58.1 57.9 1362-1366 bp 1.0 71.9
rps16-trnQ-UUG_p3 TCATGTCCTTCAAGTCGCAC GGTTCGAATCCTTCCGTCC 50.0 57.9 58.2 57.9 1374-1378 bp 1.0 71.4
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC GTTCGAATCCTTCCGTCCC 50.0 57.9 58.2 57.9 1373-1377 bp 1.0 71.4
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TTCGAATCCTTCCGTCCCA 45.0 52.6 58.1 58.3 1361-1365 bp 1.0 69.8