Hotspot

psbA-trnK-UUU

LSC · 1144-1387 · 244 bp

Back to Rohdea

Pi 0.00751
MarkerSeek score 57.40
Primer available yes
Label psbA-trnK-UUU

Feature Metadata

label_name
psbA-trnK-UUU
feature_type
igs
region
LSC
length_bp
244
variable_sites
3
pi
0.00751
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-trnK-UUU_p1 GGGGATCATCAAAACACCGA GGTTTGGGAAGGGGTCTTTT 50.0 50.0 57.9 57.9 451 bp 1.0 78.8
psbA-trnK-UUU_p2 TGGGGATCATCAAAACACCG GGTTTGGGAAGGGGTCTTTT 50.0 50.0 58.2 57.9 452 bp 1.0 78.4
psbA-trnK-UUU_p3 TAAAGACGGTTTTCGGTGCT GGTTTGGGAAGGGGTCTTTT 45.0 50.0 57.8 57.9 420 bp 1.0 77.8
psbA-trnK-UUU_p4 AACACCGAACCATCCAATGT GGTTTGGGAAGGGGTCTTTT 45.0 50.0 57.7 57.9 439 bp 1.0 77.5
psbA-trnK-UUU_p5 GCTTGTACTTTCGCGTCTCT GGTTTGGGAAGGGGTCTTTT 50.0 50.0 58.3 57.9 370 bp 1.0 77.5