Hotspot

matK

LSC · 2048-3568 · 1521 bp

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Pi 0.00131

MarkerSeek score 59.50

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1521
variable_sites: 2
pi: 0.00131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TCAATCCTTACTGCATGAACA	TGTTTTGACCGTATCGCACT	38.1	45.0	55.2	57.8	1696 bp	1.0	48.6
matK_p2	CCTTACTGCATGAACATTTCAGA	TGTTTTGACCGTATCGCACT	39.1	45.0	57.1	57.8	1691 bp	1.0	47.8
matK_p3	TCCTTACTGCATGAACATTTCAG	TGTTTTGACCGTATCGCACT	39.1	45.0	57.1	57.8	1692 bp	1.0	47.8
matK_p4	CAGTTGATTCAATCCTTACTGCA	TGTTTTGACCGTATCGCACT	39.1	45.0	57.1	57.8	1704 bp	1.0	47.7
matK_p5	TCCTTACTGCATGAACATTTCA	TGTTTTGACCGTATCGCACT	36.4	45.0	56.0	57.8	1692 bp	1.0	46.8