Hotspot

ccsA

SSC · 117625-118581 · 957 bp

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Pi 0.00104

MarkerSeek score 59.40

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 957
variable_sites: 1
pi: 0.00104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GCGGCATGGGATCTTCTAAA	TCGTAAAGCCCTTTCTGCAA	50.0	45.0	58.0	57.7	1094 bp	1.0	77.7
ccsA_p2	GCGGCATGGGATCTTCTAAA	TTCGTAAAGCCCTTTCTGCA	50.0	45.0	58.0	57.7	1095 bp	1.0	77.7
ccsA_p3	GCGGCATGGGATCTTCTAAA	CGTCGGCTTACACGAAGTTT	50.0	50.0	58.0	58.9	1144 bp	1.0	72.9
ccsA_p4	ATTCCCGATTTCCATTCCGT	TCGTAAAGCCCTTTCTGCAA	45.0	45.0	57.3	57.7	1044 bp	1.0	72.1
ccsA_p5	ATTCCCGATTTCCATTCCGT	TTCGTAAAGCCCTTTCTGCA	45.0	45.0	57.3	57.7	1045 bp	1.0	72.1