Hotspot

ycf1-ndhF

SSC · 113350-113391 · 42 bp

Back to Rodgersia

Pi 0.0476

MarkerSeek score 79.90

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 42
variable_sites: 2
pi: 0.0476
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	AAAACCTCTTGTGACCCGTC	GGTGGGCGCATTTCTTCTTA	50.0	50.0	58.0	58.5	176-184 bp	1.0	76.3
ycf1-ndhF_p2	CTTGTGACCCGTCTTTTCGA	GGTGGGCGCATTTCTTCTTA	50.0	50.0	58.1	58.5	169-177 bp	1.0	75.5
ycf1-ndhF_p3	TCTTGTGACCCGTCTTTTCG	GGTGGGCGCATTTCTTCTTA	50.0	50.0	58.1	58.5	170-178 bp	1.0	75.5
ycf1-ndhF_p4	CCTCTTGTGACCCGTCTTTT	GGTGGGCGCATTTCTTCTTA	50.0	50.0	57.7	58.5	172-180 bp	1.0	74.5
ycf1-ndhF_p5	AAAACCTCTTGTGACCCGTC	AAAATATGTAGGGGGTGGGC	50.0	50.0	58.0	57.2	189-197 bp	1.0	74.4