Hotspot

atpH-atpI

LSC · 14597-15681 · 1085 bp

Back to Rodgersia

Pi 0.00277

MarkerSeek score 60.40

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1085
variable_sites: 3
pi: 0.00277
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	GGGGAATCTATGGAGGGTCA	45.0	55.0	58.0	57.9	1143-1144 bp	1.0	79.0
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCAGCGG	45.0	45.0	58.0	57.9	1171-1172 bp	1.0	78.9
atpH-atpI_p3	AGCGGCAGAAATCAATGGAT	GGGGAATCTATGGAGGGTCA	45.0	55.0	57.9	57.9	1135-1136 bp	1.0	78.8
atpH-atpI_p4	AGCGGCAGAAATCAATGGAT	TTTTGCAACTTTAGCAGCGG	45.0	45.0	57.9	57.9	1163-1164 bp	1.0	78.7
atpH-atpI_p5	TACCTTGACCAACTCCAGGT	GGGGAATCTATGGAGGGTCA	50.0	55.0	57.9	57.9	1199-1200 bp	1.0	78.4