Hotspot

psaC

SSC · 111218-111463 · 246 bp

Back to Rochelia

Pi 0.00352

MarkerSeek score 29.30

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 2
pi: 0.00352
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	CACGGGTTTTGTCGGTAAAA	TGATTCGTTTCTTACTAGATCGA	45.0	34.8	56.9	55.1	359 bp	1.0	40.4
psaC_p2	GCACGGGTTTTGTCGGTAAA	TGATTCGTTTCTTACTAGATCGA	50.0	34.8	59.3	55.1	360 bp	1.0	40.4
psaC_p3	CACGGGTTTTGTCGGTAAAAA	TGATTCGTTTCTTACTAGATCGA	42.9	34.8	57.6	55.1	359 bp	1.0	40.4
psaC_p4	CACGGGTTTTGTCGGTAAAA	TGATTCGTTTCTTACTAGATCGAA	45.0	33.3	56.9	55.8	359 bp	1.0	40.4
psaC_p5	CACGGGTTTTGTCGGTAAAA	TGATTCGTTTCTTACTAGATCG	45.0	36.4	56.9	53.6	359 bp	1.0	40.4