Hotspot

ndhD

SSC · 109604-111154 · 1551 bp

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Pi 0.00335

MarkerSeek score 34.60

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1551
variable_sites: 12
pi: 0.00335
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TTGTGGCGTCAATAGGCTTT	TGATACCTTGGGACGGATGT	45.0	50.0	58.1	58.1	2042-2050 bp	1.0	79.2
ndhD_p2	ATCCGCCGTTTTTCTTCAGT	TGATACCTTGGGACGGATGT	45.0	50.0	58.1	58.1	2369-2377 bp	1.0	79.0
ndhD_p3	AATTGTGGCGTCAATAGGCT	TGATACCTTGGGACGGATGT	45.0	50.0	57.9	58.1	2044-2052 bp	1.0	78.9
ndhD_p4	ATTGTGGCGTCAATAGGCTT	TGATACCTTGGGACGGATGT	45.0	50.0	57.9	58.1	2043-2051 bp	1.0	78.9
ndhD_p5	GTGTAAATCGACGCAAACCG	TGATACCTTGGGACGGATGT	50.0	50.0	58.1	58.1	1893 bp	1.0	78.6