Hotspot

clpP

LSC · 65759-67687 · 1929 bp

Back to Rochelia

Pi 0.00176

MarkerSeek score 53.20

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 1929
variable_sites: 9
pi: 0.00176
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TGCGTGAAATCCATGTATGT	ACGTTTCCACATCAAAGTGA	40.0	40.0	55.4	55.5	2006-2011 bp	1.0	40.6
clpP_p2	TGCGTGAAATCCATGTATGTT	ACGTTTCCACATCAAAGTGA	38.1	40.0	56.2	55.5	2006-2011 bp	1.0	40.6
clpP_p3	TTGCGTGAAATCCATGTATGT	ACGTTTCCACATCAAAGTGA	38.1	40.0	56.2	55.5	2007-2012 bp	1.0	40.6
clpP_p4	TGCGTGAAATCCATGTATGT	ACGTTTCCACATCAAAGTGAA	40.0	38.1	55.4	56.3	2006-2011 bp	1.0	40.6
clpP_p5	TGCGTGAAATCCATGTATGTT	ACGTTTCCACATCAAAGTGAA	38.1	38.1	56.2	56.3	2006-2011 bp	1.0	40.6