Hotspot

ndhF-rpl32

SSC · 106969-107552 · 584 bp

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Pi 0.00548

MarkerSeek score 43.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 584
variable_sites: 9
pi: 0.00548
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCCCACATACGACGAAGATT	TGTCCAATACCCCCTCCTTT	50.0	50.0	58.1	57.9	767-788 bp	1.0	84.0
ndhF-rpl32_p2	GCCCACATACGACGAAGATT	TGCCGCACAACAAAACTTTT	50.0	40.0	58.1	57.9	835-856 bp	1.0	83.8
ndhF-rpl32_p3	GCCCACATACGACGAAGATT	TTGCCGCACAACAAAACTTT	50.0	40.0	58.1	57.9	836-857 bp	1.0	83.8
ndhF-rpl32_p4	GCCCACATACGACGAAGATT	TTTGCCGCACAACAAAACTT	50.0	40.0	58.1	57.9	837-858 bp	1.0	83.8
ndhF-rpl32_p5	GCCCACATACGACGAAGATT	TAGCGGCTTGTCTTCAACTC	50.0	50.0	58.1	57.9	1437-1453 bp	1.0	82.9