Hotspot

cemA-petA

LSC · 58402-58613 · 212 bp

Back to Rochelia

Pi 0.0173

MarkerSeek score 42.80

Primer available yes

Label cemA-petA

Feature Metadata

label_name: cemA-petA
feature_type: igs
region: LSC
length_bp: 212
variable_sites: 11
pi: 0.0173
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA-petA_p1	GGTGGAATCTCGGGAAATCC	CCACTTGACGTTCATCCGAT	55.0	50.0	58.0	58.0	1505-1510 bp	1.0	80.6
cemA-petA_p2	GGTGGAATCTCGGGAAATCC	TTGACGTTCATCCGATGCAT	55.0	45.0	58.0	58.0	1501-1506 bp	1.0	80.5
cemA-petA_p3	ATAGAGTCGACGAATGGGGT	CCACTTGACGTTCATCCGAT	50.0	50.0	57.9	58.0	1670-1675 bp	1.0	80.3
cemA-petA_p4	TCGGATTCCATTCACCACAC	CCACTTGACGTTCATCCGAT	50.0	50.0	57.9	58.0	1133-1138 bp	1.0	80.3
cemA-petA_p5	ATAGAGTCGACGAATGGGGT	TTGACGTTCATCCGATGCAT	50.0	45.0	57.9	58.0	1666-1671 bp	1.0	80.2