Hotspot

ycf4-cemA

LSC · 56873-57711 · 839 bp

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Pi 0.00318
MarkerSeek score 49.10
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
839
variable_sites
7
pi
0.00318
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 CATTCCCTTGACCCGTACTG AAGAGGAGTGAATGCTTTCT 55.0 40.0 58.0 54.3 972-1008 bp 1.0 53.0
ycf4-cemA_p2 CATTCCCTTGACCCGTACTG AGAAAAGAGGAGTGAATGCT 55.0 40.0 58.0 54.3 976-1012 bp 1.0 53.0
ycf4-cemA_p3 CATTCCCTTGACCCGTACTG AGAGGAGTGAATGCTTTCTT 55.0 40.0 58.0 54.3 971-1007 bp 1.0 53.0
ycf4-cemA_p4 TTCCCTTGACCCGTACTGAT AAGAGGAGTGAATGCTTTCT 50.0 40.0 58.1 54.3 970-1006 bp 1.0 52.8
ycf4-cemA_p5 TTCCCTTGACCCGTACTGAT AGAGGAGTGAATGCTTTCTT 50.0 40.0 58.1 54.3 969-1005 bp 1.0 52.8