Hotspot

rbcL-psaI

LSC · 54424-55816 · 1393 bp

Back to Rochelia

Pi 0.00466

MarkerSeek score 46.20

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1393
variable_sites: 16
pi: 0.00466
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 46.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	TCGAGGAAGTGGATAAATTGGA	GCCATTGAAATTGCCGGAAA	40.9	45.0	56.9	57.9	973-1494 bp	1.0	59.4
rbcL-psaI_p2	ACATTTACTTTCGAGGAAGTGGA	GCCATTGAAATTGCCGGAAA	39.1	45.0	57.8	57.9	983-1504 bp	1.0	58.4
rbcL-psaI_p3	CACATTTACTTTCGAGGAAGTGG	GCCATTGAAATTGCCGGAAA	43.5	45.0	57.7	57.9	984-1505 bp	1.0	58.0
rbcL-psaI_p4	TCGAGGAAGTGGATAAATTGGA	GAAGCCATTGAAATTGCCGG	40.9	50.0	56.9	58.4	976-1497 bp	1.0	57.3
rbcL-psaI_p5	TTCGAGGAAGTGGATAAATTGGA	GCCATTGAAATTGCCGGAAA	39.1	45.0	57.6	57.9	974-1495 bp	1.0	56.8