Hotspot

trnT-GGU-psbD

LSC · 30283-31444 · 1162 bp

Back to Rochelia

Pi 0.00172
MarkerSeek score 57.00
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1162
variable_sites
6
pi
0.00172
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GGACCAGCCTACAAAAACGA 55.0 50.0 58.1 58.1 1293-1322 bp 1.0 81.0
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GGACCAGCCTACAAAAACGA 50.0 50.0 58.1 58.1 1296-1325 bp 1.0 80.8
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1283-1312 bp 1.0 79.9
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1286-1315 bp 1.0 79.7
trnT-GGU-psbD_p5 GGCGTAAGTCATCGGTTCAA GGACCAGCCTACAAAAACGA 50.0 50.0 58.3 58.1 1270-1299 bp 1.0 79.3