Hotspot

trnC-GCA-petN

LSC · 27488-28270 · 783 bp

Back to Rochelia

Pi 0.00213

MarkerSeek score 50.70

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 783
variable_sites: 5
pi: 0.00213
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	846-873 bp	1.0	81.4
trnC-GCA-petN_p2	GTATCATTTTGGCGGCATGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	57.9	58.0	898-925 bp	1.0	81.0
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	CGCTTCTTCCCCATACTACG	50.0	55.0	58.1	57.9	894-921 bp	1.0	80.3
trnC-GCA-petN_p4	GTATCATTTTGGCGGCATGG	CGCTTCTTCCCCATACTACG	50.0	55.0	57.9	57.9	946-973 bp	1.0	79.8
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.4	58.0	864-891 bp	1.0	79.2