Hotspot

atpH-atpI

LSC · 13031-14124 · 1094 bp

Back to Rochelia

Pi 0.00305

MarkerSeek score 49.90

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1094
variable_sites: 10
pi: 0.00305
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACAGAAGCGGCAGAAATCA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.1	57.9	1174-1185 bp	1.0	79.5
atpH-atpI_p2	AACAGAAGCGGCAGAAATCA	TAGCTGCGGCGTATATAGGT	45.0	50.0	58.1	58.2	1163-1174 bp	1.0	79.2
atpH-atpI_p3	TACCCTCTACAGCTTGACCC	TTTTGCAACTTTAGCTGCGG	55.0	45.0	58.1	57.9	1259-1270 bp	1.0	79.1
atpH-atpI_p4	GCGATACCCTCTACAGCTTG	TTTTGCAACTTTAGCTGCGG	55.0	45.0	57.9	57.9	1263-1274 bp	1.0	79.1
atpH-atpI_p5	AGCCAATCCAGCAGCAATAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	57.9	57.9	1192-1203 bp	1.0	79.1