Hotspot

ycf1

SSC · 107595-112673 · 5079 bp

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Pi 0.0031

MarkerSeek score 54.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5079
variable_sites: 37
pi: 0.0031
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATCCTTTTGGTCCTCCTCGA	CCATTTCGCCATTTTTGTGGA	50.0	42.9	58.0	58.0	1003 bp	1.0	72.1
ycf1_p2	ATCCTTTTGGTCCTCCTCGA	TCCATTTCGCCATTTTTGTGG	50.0	42.9	58.0	58.0	1004 bp	1.0	72.1
ycf1_p3	TGGTCCTCCTCGAAAAACAC	CCATTTCGCCATTTTTGTGGA	50.0	42.9	57.8	58.0	996 bp	1.0	70.5
ycf1_p4	TGGTCCTCCTCGAAAAACAC	TCCATTTCGCCATTTTTGTGG	50.0	42.9	57.8	58.0	997 bp	1.0	70.5
ycf1_p5	TCCTTTTGGTCCTCCTCGAA	CCATTTCGCCATTTTTGTGGA	50.0	42.9	58.3	58.0	1002 bp	1.0	70.3