Hotspot

rpl20

LSC · 67706-68068 · 363 bp

Back to Robinsonia

Pi 0.00147

MarkerSeek score 26.10

Primer available yes

Label rpl20

Feature Metadata

label_name: rpl20
feature_type: gene
region: LSC
length_bp: 363
variable_sites: 1
pi: 0.00147
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20_p1	TTTTTATTCGACCCTCCCGG	ACTAGGAAAGGGGCAAAAGA	50.0	45.0	57.6	56.3	498 bp	1.0	63.6
rpl20_p2	TCCCTTTAAGCGGTGGATTC	ACTAGGAAAGGGGCAAAAGA	50.0	45.0	57.6	56.3	459 bp	1.0	63.5
rpl20_p3	CCTTTAAGCGGTGGATTCCT	ACTAGGAAAGGGGCAAAAGA	50.0	45.0	57.6	56.3	457 bp	1.0	63.5
rpl20_p4	CTCCCTTTAAGCGGTGGATT	ACTAGGAAAGGGGCAAAAGA	50.0	45.0	57.6	56.3	460 bp	1.0	63.5
rpl20_p5	TTTTATTCGACCCTCCCGGA	ACTAGGAAAGGGGCAAAAGA	50.0	45.0	58.4	56.3	497 bp	1.0	63.4