Hotspot

trnL-UAG-rpl32

SSC · 122157-122993 · 837 bp

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Pi 0.00232
MarkerSeek score 55.20
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
837
variable_sites
4
pi
0.00232
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC TGGCAGCGTTAAAAGCTTTG 50.0 45.0 58.0 57.9 947-955 bp 1.0 80.0
trnL-UAG-rpl32_p2 CAGCGTGTCTACCGATTTCA TGGCAGCGTTAAAAGCTTTG 50.0 45.0 58.0 57.9 948-956 bp 1.0 80.0
trnL-UAG-rpl32_p3 AGCGTGTCTACCGATTTCAC GGCAGCGTTAAAAGCTTTGT 50.0 45.0 58.0 57.9 946-954 bp 1.0 80.0
trnL-UAG-rpl32_p4 CAGCGTGTCTACCGATTTCA GGCAGCGTTAAAAGCTTTGT 50.0 45.0 58.0 57.9 947-955 bp 1.0 79.9
trnL-UAG-rpl32_p5 TCCTAAGAGCAGCGTGTCTA TGGCAGCGTTAAAAGCTTTG 50.0 45.0 57.9 57.9 957-965 bp 1.0 79.0