Hotspot

ycf4-cemA

LSC · 59974-60353 · 380 bp

Back to Robinsonia

Pi 0.0064

MarkerSeek score 47.30

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 380
variable_sites: 5
pi: 0.0064
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CTTGCGTGTACCAATTGAAGT	GAGAAATCCACCAGGGCAAA	42.9	50.0	57.1	58.1	478-485 bp	1.0	65.7
ycf4-cemA_p2	TTGCGTGTACCAATTGAAGT	GAGAAATCCACCAGGGCAAA	40.0	50.0	55.9	58.1	477-484 bp	1.0	64.0
ycf4-cemA_p3	TCTTGCGTGTACCAATTGAAG	GAGAAATCCACCAGGGCAAA	42.9	50.0	56.8	58.1	479-486 bp	1.0	63.5
ycf4-cemA_p4	CTTGCGTGTACCAATTGAAGT	AGAAATCCACCAGGGCAAAA	42.9	45.0	57.1	57.6	477-484 bp	1.0	63.2
ycf4-cemA_p5	TCTTGCGTGTACCAATTGAA	GAGAAATCCACCAGGGCAAA	40.0	50.0	55.6	58.1	479-486 bp	1.0	61.7