Hotspot

ndhK-ndhC

LSC · 49861-49913 · 53 bp

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Pi 0.0302

MarkerSeek score 56.60

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 53
variable_sites: 3
pi: 0.0302
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	ACCATAGAGAAGCGGCCATA	GTTTATGCATGGCGAAAGGG	50.0	50.0	58.3	57.8	211-213 bp	1.0	77.6
ndhK-ndhC_p2	ACCATAGAGAAGCGGCCATA	TGGGCAATGAGTTTCGATGT	50.0	45.0	58.3	57.8	292-294 bp	1.0	77.2
ndhK-ndhC_p3	ACCATAGAGAAGCGGCCATA	GGGGCATTGGAATGGTCTTA	50.0	50.0	58.3	57.5	193-195 bp	1.0	75.8
ndhK-ndhC_p4	CCATAGAGAAGCGGCCATAA	GTTTATGCATGGCGAAAGGG	50.0	50.0	57.2	57.8	210-212 bp	1.0	73.1
ndhK-ndhC_p5	CCATAGAGAAGCGGCCATAA	TGGGCAATGAGTTTCGATGT	50.0	45.0	57.2	57.8	291-293 bp	1.0	72.8