Hotspot

trnF-GAA-ndhJ

LSC · 47909-48600 · 692 bp

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Pi 0.00145

MarkerSeek score 54.50

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 692
variable_sites: 3
pi: 0.00145
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	50.0	50.0	58.1	56.8	792-797 bp	1.0	70.6
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	GGATAGGATGGCCTTTACGT	50.0	50.0	58.1	56.8	791-796 bp	1.0	70.6
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGATGGCC	50.0	47.6	58.1	57.7	799-804 bp	1.0	70.0
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	TTGGATAGGATGGCCTTTACG	50.0	47.6	58.1	57.5	793-798 bp	1.0	68.5
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACGT	50.0	47.6	58.1	58.6	792-797 bp	1.0	67.6