Hotspot

atpF-atpA

LSC · 28181-28263 · 83 bp

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Pi 0.0508

MarkerSeek score 83.50

Primer available yes

Label atpF-atpA

Feature Metadata

label_name: atpF-atpA
feature_type: igs
region: LSC
length_bp: 83
variable_sites: 7
pi: 0.0508
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpA_p1	ATATTGGCATATTGGGGGCG	GTCGGCTTGAATGGTTACCA	50.0	50.0	58.1	58.2	140-145 bp	1.0	85.3
atpF-atpA_p2	ATATTGGCATATTGGGGGCG	ATTTCGTCGGCTTGAATGGT	50.0	45.0	58.1	58.2	145-150 bp	1.0	85.2
atpF-atpA_p3	ATATTGGCATATTGGGGGCG	CAATGCCGTCACCTACTTGA	50.0	50.0	58.1	57.9	237-242 bp	1.0	84.4
atpF-atpA_p4	ATATTGGCATATTGGGGGCG	TCGTCGGCTTGAATGGTTAC	50.0	50.0	58.1	58.3	142-147 bp	1.0	84.4
atpF-atpA_p5	ATATTGGCATATTGGGGGCG	GCTCAATACGTTCGCGGATA	50.0	50.0	58.1	58.2	174-179 bp	1.0	84.1