Hotspot

atpH-atpF

LSC · 26537-26921 · 385 bp

Back to Robinsonia

Pi 0.00779

MarkerSeek score 50.90

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 385
variable_sites: 7
pi: 0.00779
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	CGAGGCGGAGGGAAAAATAA	TGGCCCAAAGAAACGAAAGA	50.0	45.0	58.0	57.9	540-541 bp	1.0	80.4
atpH-atpF_p2	CGAGGCGGAGGGAAAAATAA	ATTAAACCCGAAACTCCCGG	50.0	50.0	58.0	57.9	571-572 bp	1.0	79.9
atpH-atpF_p3	CGAGGCGGAGGGAAAAATAA	GTGGCCCAAAGAAACGAAAG	50.0	50.0	58.0	57.9	541-542 bp	1.0	79.8
atpH-atpF_p4	CGAGGCGGAGGGAAAAATAA	AGTGGCCCAAAGAAACGAAA	50.0	45.0	58.0	58.2	542-543 bp	1.0	79.1
atpH-atpF_p5	GCCTGGTTGTAGCATTAGCA	TGGCCCAAAGAAACGAAAGA	50.0	45.0	58.2	57.9	469-470 bp	1.0	78.9