Hotspot

ycf1

SSC · 124038-129374 · 5337 bp

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Pi 0.0139

MarkerSeek score 47.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5337
variable_sites: 74
pi: 0.0139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTGATCCACACGATGTTCA	CGTATCTTCTTTGTCACAGGC	40.0	47.6	55.3	57.0	323 bp	1.0	44.4
ycf1_p2	CTTTTGATCCACACGATGTTCA	CGTATCTTCTTTGTCACAGGC	40.9	47.6	57.3	57.0	325 bp	1.0	44.0
ycf1_p3	TTTTGATCCACACGATGTTCA	CGTATCTTCTTTGTCACAGGC	38.1	47.6	56.1	57.0	324 bp	1.0	42.8
ycf1_p4	TGATCCACACGATGTTCATT	CGTATCTTCTTTGTCACAGGC	40.0	47.6	55.1	57.0	321 bp	1.0	42.5
ycf1_p5	TTGATCCACACGATGTTCAT	CGTATCTTCTTTGTCACAGGC	40.0	47.6	55.1	57.0	322 bp	1.0	42.5