Hotspot

rps3-rpl2

LSC · 84327-85097 · 771 bp

Back to Robinia

Pi 0.0259

MarkerSeek score 69.20

Primer available yes

Label rps3-rpl2

Feature Metadata

label_name: rps3-rpl2
feature_type: igs
region: LSC
length_bp: 771
variable_sites: 20
pi: 0.0259
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl2_p1	CAAGTCTGAAACCAAGCGGA	GGCCCCCATTGGTAGAAAAA	50.0	50.0	58.4	58.1	926-985 bp	1.0	78.0
rps3-rpl2_p2	CAAGTCTGAAACCAAGCGGA	CTGCACTTGGACGAAGAAGT	50.0	50.0	58.4	58.1	882-941 bp	1.0	77.5
rps3-rpl2_p3	CAAGTCTGAAACCAAGCGGA	TTGGGGTTATCCTGCACTTG	50.0	50.0	58.4	57.8	893-952 bp	1.0	76.8
rps3-rpl2_p4	AAGTCTGAAACCAAGCGGAT	GGCCCCCATTGGTAGAAAAA	45.0	50.0	57.4	58.1	925-984 bp	1.0	76.7
rps3-rpl2_p5	AGTCTGAAACCAAGCGGATT	GGCCCCCATTGGTAGAAAAA	45.0	50.0	57.4	58.1	924-983 bp	1.0	76.7