Hotspot

rpl36-rps8

LSC · 80402-80841 · 440 bp

Back to Robinia

Pi 0.025

MarkerSeek score 69.80

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 440
variable_sites: 11
pi: 0.025
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	ATTTTACGAACGGAGGCTCC	AGGAGGGATGGGGATTGTAA	50.0	50.0	58.0	57.4	575-580 bp	1.0	76.5
rpl36-rps8_p2	ATTTTACGAACGGAGGCTCC	TTAGGAGGGATGGGGATTGT	50.0	50.0	58.0	57.4	577-582 bp	1.0	76.5
rpl36-rps8_p3	TACGAACGGAGGCTCCTATT	AGGAGGGATGGGGATTGTAA	50.0	50.0	57.9	57.4	571-576 bp	1.0	76.2
rpl36-rps8_p4	TACGAACGGAGGCTCCTATT	TTAGGAGGGATGGGGATTGT	50.0	50.0	57.9	57.4	573-578 bp	1.0	76.2
rpl36-rps8_p5	TTACGAACGGAGGCTCCTAT	AGGAGGGATGGGGATTGTAA	50.0	50.0	57.9	57.4	572-577 bp	1.0	76.2