Hotspot

trnW-CCA-trnP-UGG

LSC · 66817-67012 · 196 bp

Back to Robinia

Pi 0.051

MarkerSeek score 76.70

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 196
variable_sites: 10
pi: 0.051
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	GCGTTCTACCGAACTGAACT	GGGTACAAAATGTCACGGGT	50.0	50.0	57.9	58.1	263-277 bp	1.0	81.7
trnW-CCA-trnP-UGG_p2	GCGTTCTACCGAACTGAACT	GTCACGGGTTCAAATCCTGT	50.0	50.0	57.9	58.1	252-266 bp	1.0	81.7
trnW-CCA-trnP-UGG_p3	GCGTTCTACCGAACTGAACT	CACGGGTTCAAATCCTGTCA	50.0	50.0	57.9	58.1	250-264 bp	1.0	81.7
trnW-CCA-trnP-UGG_p4	GCGTTCTACCGAACTGAACT	TGTCACGGGTTCAAATCCTG	50.0	50.0	57.9	58.1	253-267 bp	1.0	81.7
trnW-CCA-trnP-UGG_p5	GCGTTCTACCGAACTGAACT	TGGGTACAAAATGTCACGGG	50.0	50.0	57.9	58.1	264-278 bp	1.0	81.6