Hotspot

petA-psbJ

LSC · 62891-64103 · 1213 bp

Back to Robinia

Pi 0.0124

MarkerSeek score 66.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1213
variable_sites: 15
pi: 0.0124
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TTACGTGTCCAAGGTCTCCT	ATTCCGGATTGGGATCATCC	50.0	50.0	58.0	57.1	1353-1356 bp	1.0	74.2
petA-psbJ_p2	GTTACGTGTCCAAGGTCTCC	ATTCCGGATTGGGATCATCC	55.0	50.0	57.9	57.1	1354-1357 bp	1.0	73.6
petA-psbJ_p3	TGTCCAAGGTCTCCTGTTCT	ATTCCGGATTGGGATCATCC	50.0	50.0	57.9	57.1	1348-1351 bp	1.0	73.4
petA-psbJ_p4	GTGTCCAAGGTCTCCTGTTC	ATTCCGGATTGGGATCATCC	55.0	50.0	57.8	57.1	1349-1352 bp	1.0	72.8
petA-psbJ_p5	GGTCTCCTGTTCTTCTTGGC	ATTCCGGATTGGGATCATCC	55.0	50.0	58.2	57.1	1341-1344 bp	1.0	72.7