Hotspot

psaA-ycf3

LSC · 51648-52507 · 860 bp

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Pi 0.0245

MarkerSeek score 72.80

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 860
variable_sites: 21
pi: 0.0245
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGCCCATTCCTCGAAAGAAG	AGCCCTTACCCCTGGTAATT	50.0	50.0	57.9	58.0	1008-1042 bp	1.0	81.0
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	AGCCCTTACCCCTGGTAATT	50.0	50.0	57.9	58.0	1007-1041 bp	1.0	81.0
psaA-ycf3_p3	TGCCCATTCCTCGAAAGAAG	AGCTATAGCCCTTACCCCTG	50.0	55.0	57.9	58.0	1014-1048 bp	1.0	80.8
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	AGCTATAGCCCTTACCCCTG	50.0	55.0	57.9	58.0	1013-1047 bp	1.0	80.8
psaA-ycf3_p5	GAGAAATGACCGGGTTTTGC	AGCCCTTACCCCTGGTAATT	50.0	50.0	57.7	58.0	1025-1059 bp	1.0	79.1